منابع مشابه
Gel Chromatographic Analysis of Nascent Globin Chains
A gel chromatographic analytical procedure using Bio-Gel A-OSm in 6 M guanidine HCl-0.1 M Z-mercaptoethanol has been developed and standardized. The procedure permits the molecular weight of peptides in the size range of 2 to 146 amino acids in length to be established from the distribution coefficient of the peptide on the gel column. This procedure has been applied to an analysis of the size ...
متن کاملDelayed removal of N-terminal methionine from nascent globin chains in sickle-cell anemia reticulocytes.
Synthetic alphaT1 and betaT1, the N-terminal tryptic peptides of alpha-chain and beta-chain of hemoglobin, and MetalphaT1 and MetbetaT1, peptides in which N-terminal methionyl residues are peptide-bonded to alphaT1 and betaT1, were prepared by the solid-state method of Merrifield. These synthetic peptides were used to establish conditions for chromatographic purification and analysis. When tryp...
متن کاملSynthesis of globin chains in sickle -thalassemia.
In five patients with sickle beta-thalassemia there was balanced alpha- and beta-globin synthesis in the bone marrow and decreased total beta-chain synthesis relative to that of alpha-chain in the peripheral blood. These findings are similar to those in patients with simple beta-thalassemia trait. Despite a range of hemoglobin concentrations from 6.8 to 12.5 g/100 ml in the patients with sickle...
متن کاملMonoclonal antibodies specific for globin chains.
Six monoclonal antibodies specific for human globin chains are described. They are produced by stable clones obtained by raising hybridomas using cells of mice immunized with either adult or fetal hemoglobin. Characterization of the antibodies included testing against tetrameric human and other animal hemoglobins, isolated hemoglobin chains, and when indicated, cyanogen bromide fragments. Monoc...
متن کاملGlobin Chains by a Murine Monoclonal Antibody
Human embryonic e globin chains were isolated from lysates of cultured human leukemic K562 cells induced with hemin. Mice were immunized with globin chains, and a murine hybridoma cell line was established that secreted monoclonal anti-c globin antibody. A sensitive and specific slot blot immunoassay for e globin chains has been developed. #{128} Globin chains were shown to be present in hemoly...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1974
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)42459-9